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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00031003:hlh_1_enriched

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  • Comments on WBPaper00031003:hlh_1_enriched (0)

  • Overview

    WBPaper00031003:hlh_1_enriched

    Species:
    Caenorhabditis elegans
    Attribute of Microarray experiment:
    WBPaper00031003:HLH-1_induction_0_hour_A
    WBPaper00031003:HLH-1_induction_0_hour_B
    WBPaper00031003:HLH-1_induction_0_hour_C
    WBPaper00031003:HLH-1_induction_2_hour_A
    WBPaper00031003:HLH-1_induction_2_hour_B
    WBPaper00031003:HLH-1_induction_2_hour_C
    WBPaper00031003:HLH-1_induction_4_hour_A
    WBPaper00031003:HLH-1_induction_4_hour_B
    WBPaper00031003:HLH-1_induction_4_hour_C
    WBPaper00031003:HLH-1_induction_6_hour_A
    WBPaper00031003:HLH-1_induction_6_hour_B
    WBPaper00031003:HLH-1_induction_6_hour_C
    WormBase ID:
    WBPaper00031003:hlh_1_enriched
    Genes enriched in HLH-1 heat shock dataset.

    Algorithm:

    A two-class unpaired analysis was performed to identify genes that are elevated 1.7-fold or greater when compared with the reference for each dataset, at a false discovery rate of 1.8% or less for M0 and 1.2% or less for the M24 datasets.

    Remarks:

  • References

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      • Journal article

      1

    1 reference found
    The embryonic muscle transcriptome of Caenorhabditis elegans.
    Journal article
    Genome Biol
    2007

    ABSTRACT: BACKGROUND: The force generating mechanism of muscle is evolutionarily ancient; the fundamental structural and functional components of the sarcomere are common to motile animals throughout phylogeny. Recent evidence suggests that the transcription factors that regulate muscle development are also conserved. Thus, a comprehensive description of muscle gene expression in a simple model organism should define a basic muscle transcriptome that is also found in animals with more complex body plans. To this end, we have applied Micro-Array Profiling of C. elegans Cells (MAPCeL) to muscle cell populations extracted from developing C. elegans embryos. RESULTS: Fluorescence Activated Cell Sorting (FACS) was used to isolate myo-3::GFP-positive muscle cells, and their cultured derivatives, from dissociated early C. elegans embryos. Microarray analysis identified 7,070 expressed genes, 1,312 of which are enriched in the myo-3::GFP positive cell population relative to the average embryonic cell. The muscle-enriched gene set was validated by comparisons to known muscle markers, independently derived expression data, and GFP reporters in transgenic strains. These results confirm the utility of MAPCeL for cell type-specific expression profiling and reveal that 60% of these transcripts have human homologs. CONCLUSIONS: This study provides a comprehensive description of gene expression in developing C. elegans embryonic muscle cells. The finding that over half of these muscle-enriched transcripts encode proteins with human homologs suggests that mutant analysis of these genes in C. elegans could reveal evolutionarily conserved models of muscle gene function with ready application to human muscle pathologies.

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  • Regulation

    Regulated by Gene:
    Regulated by Treatment:
    heat-shock hlh-1
    Regulated by Molecule:

  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    embryo CeThe whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.
    GO terms:
    Processes:
    Processes Definition
    Muscular system development and organizationThe coordinated specification and functional assemblage of cells and tissues into the contractile organ system in the animal. C. elegans muscles are of two types: single sarcomere with focal attachment points at the ends (alimentary system and sex muscles) and obliquely striated muscles with many sarcomeres and no one substantial focal attachment point (body-wall muscles). Components of C. elegans muscles are similar to other animals and include heavy and light-chain myosin, actin, tropomyosin, troponin-like proteins, and paramyosin. Unlike other muscle systems, C. elegans muscles send neuron-like processes to neuropils that contain motor neuron axons rather than motor neurons sending axons to innervate the muscle. Contractile tissue is found throughout C. elegans and is required for locomotion (body wall muscle), eating (pharyngeal muscle), egg laying (vulval and uterine muscles, and gonad sheath), male mating (male tail muscles), and defecation (enteric muscles).