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Species » C. elegans(Genome assembly: WBcel235)

Expression cluster » WBPaper00005943:L4_specific

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  • Overview

    WBPaper00005943:L4_specific

    Species:
    Caenorhabditis elegans
    WormBase ID:
    WBPaper00005943:L4_specific
    Life stage specific marker proteins according to proteomic analysis. L4 specific.

    Algorithm:

    Database searches (MSDB, SWISS-PROT, and Wormpep databases) were performed using the Mascot software 1.8 (Matrix Science, London, UK) with carboxamidomethylation of cysteins set as fixed modifications and methionine oxidations set as variable modifications. Positive identification was achieved only when the quality criteria, 50 - 70 ppm mass accuracy and 1 or 2 miscleavage sites were met at significant probability score (usually above 100) and nearly all signals of the spectrum were assigned to the protein or mixture of proteins identified.

    Remarks:

  • References

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      • Journal article

      1

    1 reference found
    Profiling stage-dependent changes of protein expression in Caenorhabditis elegans by mass spectrometric proteome analysis leads to the identification of stage-specific marker proteins.
    Journal article
    Electrophoresis
    2003

    Proteome maps obtained by synchronization of the wild-type Caenorhabditis elegans development reflected stage-dependent molecular differences and revealed dynamic cytoskeletal processes during ontogenesis. Distinct protein spots that may function as molecular markers for the corresponding developmental stages were mass spectrometrically identified. The amount of the Cu2+-Zn2+ superoxide dismutase (CE23550) and an aspartyl proteinase (CE21681) was highest in the first larval stage (L1) and decreased during the ontogenesis from the first larval stage to the adult. Tropomyosin III (CE29059) was prominently present in the first and second larval stage (L1/L2). Abundances of actin 1 or 4 (CE12358 or CE13148) and tropomyosin I (CE28782) were particularly high in multiple spots in the third larval stage (L3). Interestingly, the amount of DIM-1 protein (CE27706), reflected by two spots, was the lowest in this stage. A particular splicing factor (CE31089) was detected only in the fourth larval stage (L4), whereas a spot with high abundance representing the cuticle collagen (CE02272) was only found highly expressed in adult animals (A). In addition, a Ca2+- binding protein (CE12368) and one protein spot which has not yet been identified, both reached their maximal spot intensities in the adult stage (A). Moreover, the ASP-1, CCT-5, GPD-1, GPD-2, HSP-6, HSP-16.2, LEC-2, LEC-2, LIN-53, LMN-1, MDH-1, NUD-1, RPA-0, RSP-12, SOD-1, TBB-1, TBB-2, TMY-1, UNC-60, and VIT-2 proteins for which mutants are available and two still unidentified protein spots which were present in all developmental stages, have been reproducibly localized in proteome maps of distinct

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  • Associations

    Anatomy Terms:
    Life Stages:
    Life Stages Definition
    L4 larva CeThe fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.
    GO terms:
    Processes:

  • Regulation

    No regulation data for WBPaper00005943:L4_specific
    Regulated by Gene:
    Regulated by Treatment:
    Regulated by Molecule: