Silva, Aline [class:all]
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35 results (0.009 seconds)
- paper:
- person: Aline de Castro Silva Federal University of Pampa; Uruguaiana, Brazil; UNIPAMPA
- person: Aline Lobao da Silva State University of Ceara; Fortaleza, Brazil
- person: Aline F. da Silva Federal University of Santa Maria; Santa Maria, Brazil
[
International Worm Meeting,
2021] Metal nanoparticles (NPs) are the most studied nanomaterials today. From a biomedical point of view, hybrid metallic NPs are very promising when compared to monofunctional NPs, as they combine characteristics and functions that alone cannot be achieved, such as Fe3O4@Ag-NPs (magnetite NPs). In addition, green synthesis has been pursued in order to reduce environmental impacts due to solvents used in the reactions. However, little is known about Fe3O4@Ag synthesized in a biogenic manner using the extract of Camelia sinensis (green tea), requiring reliable and reproducible studies that certify the biosafety of these nanomaterials and guarantee the safety for users and for the environment. A promising model for nanotoxicological analysis is Caenorhabditis elegans, as it is invertebrate, transparent, has a short life cycle and is easy to manipulate and genetically manipulate. In this sense, the present work aimed to evaluate the toxicity of Fe3O4@Ag-NPs synthesized in a biogenic way in C. elegans. N2 (wild type), CL2166 [(pAF15) gst-4p :: GFP :: NLS] and TJ356 [daf-16p :: daf-16a / b :: GFP + rol-6 (su1006)] were used. The animals were submitted to treatment at the first larval stage (L1) in an acute manner (30 minutes) at concentrations of 1, 5, 10, 50 and 100 mug / mL. The survival rate, progeny size, egg laying, number of eggs inside the worm, translocation of the DAF-16 transcription factor and glutathione-S-transferase (GST-4) expression were analyzed. We observed that the acute exposure to Fe3O4@Ag-NPs decreased the survival rate of C. elegans, in addition to decreasing reproductive parameters such as progeny size and egg laying. In addition, the treated worms showed greater translocation of DAF-16 from the cytoplasm to the cell nucleus, indicating possible activation of antioxidant enzymes in response to damage. An increase in GST-4 expression was also observed, which suggests a detoxification mechanism against xenobiotics. This study indicates that Fe3O4@Ag-NPs induces reprotoxicity in C. elegans and that there is a modulation of the transcription factor DAF-16 with its translocation into the cell nucleus and the consequent activation of detoxification processes, as observed in this study, with increased expression of the target enzyme GST-4.
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