Mello, Craig [class:all]
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200 results (0.01 seconds)
- person: Craig Mello University of Massachusetts Medical School; Worcester MA, United States of America; RNA Therapeutics Institute University of Massachusetts Medical School; Worcester MA, United States of America; Howard Hughes Medical Institute
- antibody: WBAntibody00001490
- antibody: WBAntibody00001489
- paper:
Antibody against DCR-1, provided by Dr. Craig Mello. dcr-1
Antibody against RDE-4, provided by Dr. Craig Mello. rde-4
John Yates, James Wohlschlegel, Scott Kennedy, Yanxia Bei, Ka Ming Pang, Gary Ruvkun, Daniel R Brownell, Shohei MItani, Craig C Mello, Thomas Duchaine
[
International Worm Meeting,
2005] The ribonuclease and helicase Dicer (DCR-1) plays a central role in the initiation of a variety of small RNA-mediated silencing mechanisms. It is still unclear how the activivty of the dcr-1 gene product is specifically directed toward each pathway. To begin to address these questions we set out to identify DCR-1 interacting proteins using MudPIT technology. We then systematically generated deletion alleles of each corresponding gene. Consistent with the characterized functions of DCR-1, we found proteins known to be required for microRNA maturation (ALG-1 and ALG-2), and proteins known to be required for RNAi (RDE-1, RDE-4, DRH-1, and DRH-2). We also found one novel, highly-conserved protein that appears to be required for both RNAi and development. Interestingly, at least 4 interactors appear to be suppressors of RNAi. These include two previously known proteins, ERI-1 and RRF-3, as well as two novel proteins. Lesions in the corresponding genes were identified in both our reverse and forward genetic screens, and result in an enhanced RNAi (Eri) phenotype, as well as temperature-dependent sperm defects, and a High Incidence of Males phenotypes identical to those observed in ERI-1 and RRF-3. Thus our Dicer interactors can be divided into at least three functional categories based on their associated phenotypes; 1) mediators of miRNA maturation, 2) mediators of RNAi, and 3) suppressors of RNAi. In order to further explore the functions of the new Dicer interactors we examined the accumulation of several recently identified small RNA classes in strains lacking each factor. We found that DRH-3 is required for all of the examined endogenous ORF-derived siRNAs (endo-siRNAs), most of which are expressed in the germline. The eri genes are required along with DCR-1 for the accumulation of the tiny non-coding RNAs (tncRNAs), and for small RNAs derived from an endogenous contig located on the X chromosome. In addition, the eri genes are required for a limited subset of the endo-siRNAs. These findings provide a first look at the biochemical niche of DCR-1 in C. elegans, and support a model in which specific combinations of DCR-1 interactions direct its activity toward different small RNA silencing pathways. The implication of the ERI proteins in endogenous siRNA mediated pathways suggest that their role as suppressors of RNAi may be explained by direct competition for DCR-1, or for other limiting downstream factors. TD is supported by the CIHR PDF fellowship
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