morc

MORC (mouse microrchidia) family CW-type zinc finger protein

Picture from van der Linden AM et al. (2010) PLoS Biol "Genome-wide analysis of light- and temperature-entrained circadian ...."

Expression in transgenic animals carrying stably integrated copies of an nlp-36p::gfp fusion gene during temperature entrainment (CW) and free-running (WW) conditions. Adult animals were examined under 100 magnification.

morc-1

Caenorhabditis elegans

Enables topological DNA co-entrapment activity. Involved in DNA topological change. Located in nuclear body. Expressed in somatic cell. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease axonal type 2Z. Is an ortholog of human MORC1 (MORC family CW-type zinc finger 1).

protein: MORC1

Homo sapiens

MORC family CW-type zinc finger protein 1

protein: MORC4

Homo sapiens

MORC family CW-type zinc finger protein 4

protein: MORC2

Homo sapiens

Isoform 1 of MORC family CW-type zinc finger protein 2

Picture from van der Linden AM et al. (2010) PLoS Biol "Genome-wide analysis of light- and temperature-entrained circadian ...."

Figure 9. Expression of an nlp-36p::gfp fusion gene is entrained by temperature cycles. (A) gfp expression in transgenic animals carrying stably integrated copies of an nlp-36p::gfp fusion gene during temperature entrainment (CW) and free-running (WW) conditions. Adult animals were examined under 100 magnification. (B) Quantification of intestinal nlp-36p::gfp fluorescence intensity (in arbitrary units [A.U.]) at the indicated time points during entrainment or free-running days. Wild-type transgenic animals were entrained to either WC/CC (gray bars) or CW/WW (red bars) cycles. Error bars indicate the s.e.m. Data shown are from two independent experiments with 15-20 animals at each time point. (C) nlp-36p::gfp-expressing transgenic animals were subjected to CW entrainment cycles and moved to WW conditions. After 6 h at WW, a subset of animals were subjected to a pulse of 15 C temperature for 6 h and returned to WW conditions. Intestinal fluorescence intensities in animals subjected or not subjected to the cold pulse are indicated in blue and gray, respectively. Error bars indicate the standard deviation. n = 15-20 animals each at each time point.

Bansal A et al. (2016) Small "Quasi-Continuous Wave Near-Infrared Excitation of Upconversion Nanoparticles for Optogenetic Manipulation of C. elegans."

Andersson-Engels S, Liu H, Zhang Y, Jayakumar MK, Bansal A

[Small, 2016]

Optogenetics is an emerging powerful tool to investigate workings of the nervous system. However, the use of low tissue penetrating visible light limits its therapeutic potential. Employing deep penetrating near-infrared (NIR) light for optogenetics would be beneficial but it cannot be used directly. This issue can be tackled with upconversion nanoparticles (UCNs) acting as nanotransducers emitting at shorter wavelengths extending to the UV range upon NIR light excitation. Although attractive, implementation of such NIR-optogenetics is hindered by the low UCN emission intensity that necessitates high NIR excitation intensities, resulting in overheating issues. A novel quasi-continuous wave (quasi-CW) excitation approach is developed that significantly enhances multiphoton emissions from UCNs, and for the first time NIR light-triggered optogenetic manipulations are implemented in vitro and in C. elegans. The approach developed here enables the activation of channelrhodopsin-2 with a significantly lower excitation power and UCN concentration along with negligible phototoxicity as seen with CW excitation, paving the way for therapeutic optogenetics.

Zcwpw1l1

Rattus norvegicus

Predicted to enable methylated histone binding activity. Predicted to be active in nucleus. Orthologous to human ZCWPW1 (zinc finger CW-type and PWWP domain containing 1); INTERACTS WITH dioxygen.

Zcwpw2

Rattus norvegicus

Predicted to enable methylated histone binding activity. Orthologous to human ZCWPW2 (zinc finger CW-type and PWWP domain containing 2); INTERACTS WITH bisphenol A; Tesaglitazar; (+)-catechin (ortholog).