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Resources » Paper

Xu J et al. (2023) Development "The C. elegans PUM1, 2-like RNA binding protein PUF-8 is required for robustness of the cell death fate."

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  • Comments on Xu J et al. (2023) Development "The C. elegans PUM1, 2-like RNA binding protein PUF-8 is required for robustness of the cell death fate." (0)

  • Overview

    PMID:
    37747106
    DOI:
    10.1242/dev.201167
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00066042

    Xu J, Jiang Y, Sherrard R, Ikegami K, & Conradt B (2023). The C. elegans PUM1, 2-like RNA binding protein PUF-8 is required for robustness of the cell death fate. Development. doi:10.1242/dev.201167

    During C. elegans development, 1090 somatic cells are generated of which 959 survive and 131 die, many through apoptosis. We present evidence that PUF-8, a C. elegans ortholog of the mammalian RNA binding proteins PUM1 and PUM2, is required for the robustness of this 'survival and death' pattern. We found that PUF-8 prevents the inappropriate death of cells that normally survive, and we present evidence that this anti-apoptotic activity of PUF-8 is dependent on PUF-8's ability to interact with ced-3caspase mRNA thereby repressing the activity of the pro-apoptotic ced-3caspase gene. PUF-8 also promotes the death of cells that are programmed to die, and we propose that this pro-apoptotic activity of PUF-8 may depend on PUF-8's ability to repress the expression of the anti-apoptotic ced-9Bcl-2 gene. Our results suggest that stochastic differences in the expression of genes within the apoptosis pathway can disrupt the highly reproducible and robust survival and death pattern during C. elegans development, and that PUF-8PUM1, 2 acts at the post-transcriptional level to level out these differences, thereby ensuring proper cell number homeostasis.

    We thank Barbara Conradt for curating this paper through ACKnowledge (Author Curation to Knowledgebase) 👍

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