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Resources » Paper

Horowitz BB et al. (2023) G3 (Bethesda) "A Transcriptional Cofactor Regulatory Network for the C. elegans Intestine."

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  • Comments on Horowitz BB et al. (2023) G3 (Bethesda) "A Transcriptional Cofactor Regulatory Network for the C. elegans Intestine." (0)

  • Overview

    PMID:
    37119809
    DOI:
    10.1093/g3journal/jkad096
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00065308

    Horowitz BB, Nanda S, & Walhout AJM (2023). A Transcriptional Cofactor Regulatory Network for the C. elegans Intestine. G3 (Bethesda). doi:10.1093/g3journal/jkad096

    Chromatin modifiers and transcriptional cofactors (collectively referred to as CFs) work with DNA-binding transcription factors (TFs) to regulate gene expression. In multicellular eukaryotes, distinct tissues each execute their own gene expression program for accurate differentiation and subsequent functionality. While the function of TFs in differential gene expression has been studied in detail in many systems, the contribution of CFs has remained less explored. Here we uncovered the contributions of CFs to gene regulation in the Caenorhabditis elegans intestine. We first annotated 366 CFs encoded by the C. elegans genome and assembled a library of 335 RNAi clones. Using this library, we analyzed the effects of individually depleting these CFs on the expression of 19 fluorescent transcriptional reporters in the intestine and identified 216 regulatory interactions. We found that different CFs regulate different promoters, and that both essential and intestinally expressed CFs have the greatest effects on promoter activity. We did not find all members of CF complexes acting on the same set of reporters but instead found diversity in the promoter targets of each complex component. Finally, we found that previously identified activation mechanisms for the acdh-1 promoter use different CFs and TFs. Overall, we demonstrate that CFs function specifically rather than ubiquitously at intestinal promoters and provide an RNAi resource for reverse genetic screens.

    We thank AJ Marian Walhout for curating this paper through ACKnowledge (Author Curation to Knowledgebase) 👍

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