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Resources » Paper

Yang WH et al. (2023) Curr Res Microb Sci "Impaired immune response and barrier function in GSPD-1-deficient C. elegans infected with Klebsiella pneumoniae."

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  • Comments on Yang WH et al. (2023) Curr Res Microb Sci "Impaired immune response and barrier function in GSPD-1-deficient C. elegans infected with Klebsiella pneumoniae." (0)

  • Overview

    PMID:
    36798906
    DOI:
    10.1016/j.crmicr.2023.100181
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00065034

    Yang WH, Chen PH, Chang HH, Kwok HL, Stern A, Soo PC, Chen JH, & Yang HC (2023). Impaired immune response and barrier function in GSPD-1-deficient C. elegans infected with Klebsiella pneumoniae. Curr Res Microb Sci, 4, 100181. doi:10.1016/j.crmicr.2023.100181

    gspd-1-RNAi knockdown Caenorhabditis elegans was used as an immune-compromised model to investigate the role of G6PD in host-pathogen interactions. A shorted lifespan, increased bacterial burden and bacterial translocation were observed in gspd-1-knockdown C. elegans infected with Klebsiella pneumoniae (KP). RNAseq revealed that the innate immune pathway, including clc-1 and tsp-1, was affected by gspd-1 knockdown. qPCR confirmed that tight junction (zoo-1, clc-1) and immune-associated genes (tsp-1) were down-regulated in gspd-1-knockdown C. elegans and following infection with KP. The down-regulation of antimicrobial effector lysozymes, including lys-1, lys-2, lys-7, lys-8, ilys-2 and ilys-3, was found in gspd-1-knockdown C. elegans infected with KP. Deletion of clc-1, tsp-1, lys-7, and daf-2 in gspd-1-knockdown C. elegans infected with KP abolished the shorten lifespan seen in the Mock control. GSPD-1 deficiency in C. elegans resulted in bacterial accumulation and lethality, possibly due to a defective immune response. These findings indicate that GSPD-1 has a protective role in microbial defense in C. elegans by preventing bacterial colonization through bacterial clearance.

    Authors: Yang WH, Chen PH, Chang HH, Kwok HL, Stern A, Soo PC, Chen JH, Yang HC


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