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Resources » Paper

Ruiz M et al. (2022) Biochim Biophys Acta Biomembr "A small molecule screen for paqr-2 suppressors identifies Tyloxapol as a membrane fluidizer for C. elegans and mammalian cells."

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  • Comments on Ruiz M et al. (2022) Biochim Biophys Acta Biomembr "A small molecule screen for paqr-2 suppressors identifies Tyloxapol as a membrane fluidizer for C. elegans and mammalian cells." (0)

  • Overview

    PMID:
    35588889
    DOI:
    10.1016/j.bbamem.2022.183959
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00064097

    Ruiz M, Svensk E, Einarsson E, Grahn EP, & Pilon M (2022). A small molecule screen for paqr-2 suppressors identifies Tyloxapol as a membrane fluidizer for C. elegans and mammalian cells. Biochim Biophys Acta Biomembr, 183959. doi:10.1016/j.bbamem.2022.183959

    Defects in cell membrane homeostasis are implicated in numerous disorders, including cancer, neurodegeneration and diabetes. There is therefore a need for a powerful model to study membrane homeostasis and to identify eventual therapeutic routes. The C. elegans gene paqr-2 encodes a homolog of the mammalian AdipoR1 and AdipoR2 proteins that, when mutated, causes a membrane homeostasis defect accompanied by multiple phenotypes such as intolerance to dietary saturated fatty acids, intolerance to cold and a characteristic tail tip morphology defect. We screened a compound library to identify molecules that can suppress the paqr-2 phenotypes. A single positive hit, Tyloxapol, was found that very effectively suppresses multiple paqr-2 phenotypes. Tyloxapol is a non-ionic detergent currently in use clinically as an expectorant. Importantly, we examined the potential of Tyloxapol as a fluidizer in human cells and found that it improves the viability and membrane fluidity of AdipoR2-deficient human cells challenged with palmitic acid, a membrane-rigidifying saturated fatty acid.

    We thank Marc Pilon for curating this paper through ACKnowledge (Author Curation to Knowledgebase) 👍

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