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Resources » Paper

Driesschaert, Brecht et al. (2022) MicroPubl Biol

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  • Overview

    PMID:
    35601753
    DOI:
    10.17912/micropub.biology.000573
    Status:
    Publication type:
    Micropublication, Journal_article
    WormBase ID:
    WBPaper00064030

    Driesschaert, Brecht, & Temmerman, Liesbet (2022). The Q system for conditional gene expression is leaky and lacks dynamic range in C. elegans neurons. MicroPubl Biol. doi:10.17912/micropub.biology.000573

    The Q system is a genetic tool developed to deliver spatiotemporal control over gene expression (Giles et al. 1991; Potter et al. 2010; Wei et al. 2012). Although it has already been adapted for use in C. elegans by Wei et al. in 2012, to date, the Q system has not been applied extensively in this nematode. In the relatively few available reports, it is mainly used to constitutively restrict gene expression in a spatial manner (e.g. Schild et al. 2014; Schild and Glauser 2015; Jee et al. 2016; Tolstenkov et al. 2018; Chiyoda et al. 2021), while but a handful of studies also explore the temporal aspect of the system (Matus et al. 2015; Yuan et al. 2016; Cottee et al. 2017; Hoang and Miller 2017). We aimed to apply this tool in the C. elegans nervous system to gain both spatial and temporal control over expression of a gene encoding a reporter protein that is targeted to the secretory pathway. Despite our efforts, we here report that in our hands, the Q system is not suitable for application in the neurons due to a lack of dynamic range.


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