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Resources » Paper

Hwang, Hyeonjeong et al. (2021) International Worm Meeting "Exoribonuclease ERI-1 regulates ascr#3 avoidance behavior in C. elegans"

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00063609

    Hwang, Hyeonjeong, Jo, Sujin, Oh, Seunghee, & Kim, Kyuhyung (2021). Exoribonuclease ERI-1 regulates ascr#3 avoidance behavior in C. elegans presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Small RNAs are short, non-coding RNAs that can regulate gene expression. Recent studies suggested that small RNAs pathway modulates animal behavior (Juang et al., 2013; Posner et al., 2019; Picao-Osorio et al., 2015). However, the mechanisms by which small RNAs regulate animal behavior are not fully understood. C. elegans secretes a complex pheromone mixture called ascarosides. A pheromone component, ascr#3 (asc-deltaC9, C9) has been shown to elicit avoidance behavior in wild-type hermaphrodites (Jang et al., 2012). Here, we investigate the roles of small RNAs in ascr#3-mediated avoidance behavior. First, we screened candidate mutants including rrf-3, ergo-1, drh-3, dcr-1, alg-4; alg-3, and rde-4 of which gene products are known to regulate small RNA pathways. We found that four alleles of eri-1 mutants exhibit defects in ascr#3 avoidance. We then restored the phenotype by expressing eri-1 cDNA under the control of its own promoter and pan-neuronal promoter. Next, we examined expression patterns of eri-1 and found that eri-1 is expressed in a few neurons in the head and tail. We identified that these neurons include either AVJ or AVH in the head and PVP in the tail. Besides, we found a correlation between eri-1 expression level in AVJ/AVH but not in PVP and ascr#3 avoidance; animals showing high eri-1 expression appear to exhibit increased ascr#3 avoidance and vice-versa. Moreover, we found that eri-1 expression levels in AVJ/AVH and ascr#3 avoidance are increased with maternal age. Taken together, these results indicate that exoribonuclease eri-1 regulates ascr#3 avoidance behavior, which provides an opportunity to study the roles of small RNAs in animal behavior.

    Affiliation:
    - DGIST, Daegu, Repulic of Korea


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