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Resources » Paper

Liang, Xing et al. (2021) International Worm Meeting "Growth Cone-Localized Microtubule Organizing Center Establishes Microtubule Orientation in Dendrites"

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  • Comments on Liang, Xing et al. (2021) International Worm Meeting "Growth Cone-Localized Microtubule Organizing Center Establishes Microtubule Orientation in Dendrites" (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00063070

    Liang, Xing, Kokes, Marcela, Fetter, Richard, Sallee, Maria, Moore, Adrian, Feldman, Jessica, & Shen, Kang (2021). Growth Cone-Localized Microtubule Organizing Center Establishes Microtubule Orientation in Dendrites presented in International Worm Meeting. Unpublished information; cite only with author permission.

    A polarized arrangement of neuronal microtubule arrays is the foundation of membrane trafficking and subcellular compartmentalization. Conserved among both invertebrates and vertebrates, axons contain exclusively "plus-end-out" microtubules while dendrites contain a high percentage of "minus-end-out" microtubules, the origins of which have been a mystery. Here we show that in Caenorhabditis elegans the dendritic growth cone contains a non-centrosomal microtubule organizing center, which generates minus-end-out microtubules along outgrowing dendrites and plus-end-out microtubules in the growth cone. RAB-11-positive endosomes accumulate in this region and co-migrate with the microtubule nucleation complex gamma-TuRC. The MTOC tracks the extending growth cone by kinesin-1/UNC-116-mediated endosome movements on distal plus-end-out microtubules and dynein clusters this advancing MTOC. Critically, perturbation of the function or localization of the MTOC causes reversed microtubule polarity in dendrites. These findings unveil the endosome-localized dendritic MTOC as a critical organelle for establishing axon-dendrite polarity.

    Affiliations:
    - RIKEN Center for Brain Science, Wako, Saitama
    - Department of Biology, Stanford University, Stanford, CA
    - Howard Hughes Medical Institute, Stanford University, Stanford, CA


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