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Resources » Paper

Huang X et al. (2021) Proc Natl Acad Sci U S A "A chromodomain protein mediates heterochromatin-directed piRNA expression."

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  • Comments on Huang X et al. (2021) Proc Natl Acad Sci U S A "A chromodomain protein mediates heterochromatin-directed piRNA expression." (0)

  • Overview

    PMID:
    34187893
    DOI:
    10.1073/pnas.2103723118
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00061574

    Huang X, Cheng P, Weng C, Xu Z, Zeng C, Chen X, Zhu C, Guang S, & Feng X (2021). A chromodomain protein mediates heterochromatin-directed piRNA expression. Proc Natl Acad Sci U S A, 118. doi:10.1073/pnas.2103723118

    PIWI-interacting RNAs (piRNAs) play significant roles in suppressing transposons, maintaining genome integrity, and defending against viral infections. How piRNA source loci are efficiently transcribed is poorly understood. Here, we show that in <i>Caenorhabditis elegans</i>, transcription of piRNA clusters depends on the chromatin microenvironment and a chromodomain-containing protein, UAD-2. piRNA clusters form distinct focus in germline nuclei. We conducted a forward genetic screening and identified UAD-2 that is required for piRNA focus formation. In the absence of histone 3 lysine 27 methylation or proper chromatin-remodeling status, UAD-2 is depleted from the piRNA focus. UAD-2 recruits the upstream sequence transcription complex (USTC), which binds the Ruby motif to piRNA promoters and promotes piRNA generation. Vice versa, the USTC complex is required for UAD-2 to associate with the piRNA focus. Thus, transcription of heterochromatic small RNA source loci relies on coordinated recruitment of both the readers of histone marks and the core transcriptional machinery to DNA.

    Authors: Huang X, Cheng P, Weng C, Xu Z, Zeng C, Chen X, Zhu C, Guang S, Feng X

    We thank Shouhong Guang for curating this paper through ACKnowledge (Author Curation to Knowledgebase) 👍

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