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Resources » Paper

Farhadifar R et al. (2020) Elife "Stoichiometric interactions explain spindle dynamics and scaling across 100 million years of nematode evolution."

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  • Comments on Farhadifar R et al. (2020) Elife "Stoichiometric interactions explain spindle dynamics and scaling across 100 million years of nematode evolution." (0)

  • Overview

    PMID:
    32966209
    DOI:
    10.7554/eLife.55877
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00060355

    Farhadifar R, Yu CH, Fabig G, Wu HY, Stein DB, Rockman M, Muller-Reichert T, Shelley MJ, & Needleman DJ (2020). Stoichiometric interactions explain spindle dynamics and scaling across 100 million years of nematode evolution. Elife, 9. doi:10.7554/eLife.55877

    The spindle shows remarkable diversity, and changes in an integrated fashion, as cells vary over evolution. Here, we provide a mechanistic explanation for variations in the first mitotic spindle in nematodes. We used a combination of quantitative genetics and biophysics to rule out broad classes of models of the regulation of spindle length and dynamics, and to establish the importance of a balance of cortical pulling forces acting in different directions. These experiments led us to construct a model of cortical pulling forces in which the stoichiometric interactions of microtubules and force generators (each force generator can bind only one microtubule), is key to explaining the dynamics of spindle positioning and elongation, and spindle final length and scaling with cell size. This model accounts for variations in all the spindle traits we studied here, both within species and across nematode species spanning over 100 million years of evolution.

    Authors: Farhadifar R, Yu CH, Fabig G, Wu HY, Stein DB, Rockman M, Muller-Reichert T, Shelley MJ, Needleman DJ


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