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Resources » Paper

Bhan P et al. (2019) Traffic "Characterization of TAG-63 and its role on axonal transport in C. elegans."

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  • Comments on Bhan P et al. (2019) Traffic "Characterization of TAG-63 and its role on axonal transport in C. elegans." (0)

  • Overview

    PMID:
    31622527
    DOI:
    10.1111/tra.12706
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00058719

    Bhan P, Muthaiyan Shanmugam M, Wang D, Bayansan O, Chen CW, & Wagner OI (2019). Characterization of TAG-63 and its role on axonal transport in C. elegans. Traffic. doi:10.1111/tra.12706

    Model organisms are increasingly used to study and understand how neurofilament (NF)-based neurological diseases develop. However, whether a NF homolog exists in C. elegans remains unclear. We characterize TAG-63 as a NF-like protein with sequence homologies to human NEFH carrying various coiled coils as well as clustered phosphorylation sites. TAG-63 also exhibits features of NFL such as a molecular weight of around 70 kD, the lack of KSP repeats and the ability to form 10nm filamentous structures in transmission electron micrographs. An anti-NEFH antibody detects a band at the predicted molecular weight of TAG-63 in Western blots of whole worm lysates and this band cannot be detected in tag-63 knockout worms. A transcriptional tag-63 reporter expresses in a broad range of neurons, and various anti-NFH antibodies stain worm neurons with an overlapping expression of axonal vesicle transporter UNC-104(KIF1A). Cultured neurons grow shorter axons when incubating with drugs known to disintegrate the NF network and rhodamine-labeled in vitro reconstituted TAG-63 filaments disintegrate upon drug exposure. Speeds of UNC-104 motors are diminished in tag-63 mutant worms with visibly increased accumulations of motors along axons. UNC-104/TAG-63 and SNB-1/TAG-63 not only co-localize in neurons but also revealed positive BiFC (bimolecular fluorescence assay) signals. In summary, we identified and characterized TAG-63 in C. elegans, and demonstrate that lack of this protein limits axonal transport efficiencies. Additionally, this study would aid in developing NF-related disease models in the future. This article is protected by copyright. All rights reserved.

    We thank Oliver Wagner for curating this paper through ACKnowledge (Author Curation to Knowledgebase) 👍

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