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Resources » Paper

Salazar, C. J. et al. (2019) International Worm Meeting "A novel Rab GTPase restricts dendrite branching."

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  • Comments on Salazar, C. J. et al. (2019) International Worm Meeting "A novel Rab GTPase restricts dendrite branching." (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00058379

    Salazar, C. J., Diaz-Balzac, C. A., Grant, B. D., & Bulow, H. E. (2019). A novel Rab GTPase restricts dendrite branching presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Dendrite development depends on numerous extracellular and intracellular cues to ensure proper structure and function. However, the regulatory mechanisms of dendrite development remain incompletely understood. To better understand dendrite development, we are utilizing the PVD somatosensory neuron with its highly stereotyped 'menorah'-like dendrites. The Menorin genetic pathway consists of several factors that function from different tissues to promote PVD dendrite development. While progress has been made in understanding factors that promote the formation of dendritic branches, much less is known about factors that restrict branching. During a genetic screen, we have identified a locus that encodes for a putative, uncharacterized Rab GTPase, which we name rab-X. Time course analyses determined that the number of branching points was significantly increased in adult animals. This suggests rab-X suppresses dendritic branching into adulthood. A transcriptional reporter shows expression to be in the epidermis from early embryonic through adult stages and transgenic expression of a rab-X cDNA in the epidermis is sufficient to rescue the mutant phenotype. Genetic analyses show that the Menorin pathway is largely epistatic to rab-X, indicating it may function in the same genetic pathway. In addition, we found that a rescuing N-terminal translational RAB-X fusion displays intracellular, perinuclear localization. We will present our progress to (1) determine whether mutations in rab-X affect localization of components of the Menorin pathway, to (2) determine the subcellular localization of rab-X, and to (3) determine if GTPase activity of rab-X is necessary for restricting dendritic growth.

    Affiliations:
    - Genetics, Albert Einstein College of Medicine, Bronx, NY
    - Department of Molecular Biology, Rutgers University


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