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Resources » Paper

Cao, Mengyi et al. (2019) International Worm Meeting "Multi-tissue coordination in neuropeptides regulation of Caenorhabditis elegans dauer development and behaviors."

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  • Comments on Cao, Mengyi et al. (2019) International Worm Meeting "Multi-tissue coordination in neuropeptides regulation of Caenorhabditis elegans dauer development and behaviors." (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00058271

    Cao, Mengyi, Shih, Pei Yin, & Sternberg, Paul (2019). Multi-tissue coordination in neuropeptides regulation of Caenorhabditis elegans dauer development and behaviors presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Multi-tissue coordination such as the gut-brain axis could play a crucial role in regulating animal development and behaviors. In response to harsh environmental conditions, Caenorhabditis elegans enters developmentally-arrested dauer stage through multiple signaling pathways including neuropeptide regulation. Our previous research showed that using a mutant of a pro-protein convertase chaperone (sbt-1) to inhibit FMRFamide-like neuropeptides maturation caused defects in C. elegans dauer development and behaviors. To explore how multiple tissues coordinate to control dauer-related neuropeptide maturation, we used a cGAL-UAS bipartite system to test if tissue-specific expression of mature neuropeptides could rescue dauer phenotypes. Specifically, we constructed C. elegans strains expressing a cGAL driver (fused to promoter specific to neurons, intestine, or muscles) and a UAS::sbt-1 effector in the sbt-1 mutant background and tested the ability of animals in dauer phenotypic assays. Our data show that either pan-neuronal (rab-3p) or intestinal (nlp-40p) expression of sbt-1 cDNA rescues dauer entry, while sbt-1 expression in other tested tissues (such as muscles, cholinergic and glutamatergic neurons) are not sufficient to rescue dauer entry. Our data confirmed that neuropeptide regulation in dauer development is tissue-specific and may be restricted to intestine and a small group of neurons. We will present our current progress in identifying the neuropeptides and the specific tissues (or cells) where they are produced to regulate dauer phenotypes. Our research suggests the potential of developing C. elegans dauer as a model for studying gut-brain coordination in animal behaviors.

    Affiliation:
    - BBE, California Institute of Technology, Pasadena, CA


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