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Comments on Dominguez, J. et al. (2019) International Worm Meeting "Characterization of microtubule deglutamylating enzyme function in the C. elegans germline." (0)
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Dominguez, J., Hepurker, A., John, S., Oliver, T., & Peel, N. (2019). Characterization of microtubule deglutamylating enzyme function in the C. elegans germline presented in International Worm Meeting. Unpublished information; cite only with author permission.
Microtubule glutamylation is a reversible process that regulates microtubule function by modifying them with the addition of glutamate residues. This post translational modification of the polymerized microtubule is catalyzed by a family of tubulin tyrosine ligase-like (TTLL) enzymes, while the removal of glutamate is carried out by a family of cytosolic carboxypeptidase (CCP) enzymes. C. elegans has two deglutamylating enzymes, CCPP-1 and CCPP-6, homologs of mammalian CCP1 and CCP5 respectively. Loss of mouse CCP1 function leads to neurodegeneration and reduced female fertility, whereas loss of CCP5 causes defects of the sperm flagella. C. elegans ccpp-1 mutants show hyperglutamylation and ciliary fragmentation, suggesting that CCPP-1 functions in maintaining ciliary microtubule stability (O'Hagan, 2011). Although ccpp-1(ok1821) mutants show normal brood size at 20 deg C we have identified a heat-induced reduction in brood size. This fertility defect originates in oogenesis and does not seem to result from increased germline apoptosis. In contrast, mutation of ccpp-6, does not affect brood size and the brood size defect is not worse in a ccpp-1(ok1821); ccpp-6(ok382) double mutant, suggesting that redundancy is not present in germline. In addition, we have found that ccpp-1 mutants show increased sensitivity to the microtubule destabilizing drug colchicine and to the cold. We are currently investigating the underlying cause of the ccpp-1 reduced fertility.
Affiliation:
- Department of Biology, TCNJ, Ewing, NJ