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Resources » Paper

Li, Z. et al. (2019) International Worm Meeting "Visualizing C. elegans transcripts reveals distinct dynamics of membrane-associated genes."

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  • Comments on Li, Z. et al. (2019) International Worm Meeting "Visualizing C. elegans transcripts reveals distinct dynamics of membrane-associated genes." (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00057617

    Li, Z., Zhang, P., Zhang, R., & Zhang, H. (2019). Visualizing C. elegans transcripts reveals distinct dynamics of membrane-associated genes presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Localized mRNAs contribute to targeting proteins to discrete sites and are required for diverse cellular and developmental events, including translation, cell division, cell motility and cell-fate determination. The ability to visualize mRNA in real time at high resolution in live animals are critical for full understanding the spatiotemporal dynamics of gene regulation and function. Here we report a method to visualize mRNA using PP7-PCP system in C. elegans, in which mRNAs containing bacteriophage PP7 binding sites are labeled by the PP7 coat protein fused to fluorescent reporters. Using this approach, we construct a collection of tissue-specific, differentially expressed, membrane-associated mRNA toolkit strains and detect distinct mRNA dynamics and localizations through monitoring single RNP particles. We have applied this system to dissect the cis-regulatory element for mRNA localization and explore development- and tissue-dependent mRNA-protein localization correlations. Furthermore, we attempt to use the PP7-PCP RNA labeling system to uncover the possible link between mRNA localization and epithelial polarity in C. elegans. Together our results outline a powerful methodology to monitor mRNA through its life cycle. This approach offers important advantages compared with other gene expression techniques already available in this experimentally tractable model organism. This work is supported by Multi-Year Research Grant, University of Macau (MYRG) project 2017-00082-FHS.

    Affiliation:
    - University of Macau, Taipa, Macau, China


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