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Resources » Paper

Takeuchi T et al. (2019) Glycobiology "Galectin-2 suppresses nematode development by binding to the invertebrate-specific galactose1-4fucose glyco-epitope."

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  • Comments on Takeuchi T et al. (2019) Glycobiology "Galectin-2 suppresses nematode development by binding to the invertebrate-specific galactose1-4fucose glyco-epitope." (0)

  • Overview

    PMID:
    30874734
    DOI:
    10.1093/glycob/cwz022
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00056427

    Takeuchi T, Tamura M, Ishiwata K, Hamasaki M, Hamano S, Arata Y, & Hatanaka T (2019). Galectin-2 suppresses nematode development by binding to the invertebrate-specific galactose1-4fucose glyco-epitope. Glycobiology. doi:10.1093/glycob/cwz022

    Galactose1-4Fucose (GalFuc) is a unique disaccharide found in invertebrates including nematodes. A fungal galectin CGL2 suppresses nematode development by recognizing the galactose1-4fucose epitope. The Caenorhabditis elegans galectin LEC-6 recognizes it as an endogenous ligand and the Glu67 residue of LEC-6 is responsible for this interaction. We found that mammalian galectin-2 (Gal-2) also has a comparable glutamate residue, Glu52. In the present study, we investigated the potential nematode-suppressing activity of Gal-2 using C. elegans as a model and focusing on Gal-2 binding to the GalFuc epitope. Gal-2 suppressed C. elegans development whereas its E52D mutant (Glu52 substituted by Asp), galectin-1, and galectin-3 had little effect on C. elegans growth. Lectin-staining using fluorescently-labeled Gal-2 revealed that, like CGL2, it specifically binds to the C. elegans intestine. Natural C. elegans glycoconjugates were specifically bound by immobilized Gal-2. Western blotting with anti-GalFuc antibody showed that the bound glycoconjugates had the GalFuc epitope. Frontal affinity chromatography with pyridylamine-labeled C. elegans N-glycans disclosed that Gal-2 (but not its E52D mutant) recognizes the GalFuc epitope. Gal-2 also binds to the GalFuc-bearing glycoconjugates of Ascaris and the GalFuc epitope is present in the parasitic nematodes Nippostrongylus brasiliensis and Brugia pahangi. These results indicate that Gal-2 suppresses C. elegans development by binding to its GalFuc epitope. The findings also imply that Gal-2 may prevent infestations of various parasitic nematodes bearing the GalFuc epitope.


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