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Resources » Paper

Lowrie RC (1983) Am J Trop Med Hyg "Cryopreservation of third-stage larvae of Brugia malayi and Dipetalonema viteae."

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  • Comments on Lowrie RC (1983) Am J Trop Med Hyg "Cryopreservation of third-stage larvae of Brugia malayi and Dipetalonema viteae." (0)

  • Overview

    PMID:
    6683940
    DOI:
    10.4269/ajtmh.1983.32.767
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00055202

    Lowrie RC (1983). Cryopreservation of third-stage larvae of Brugia malayi and Dipetalonema viteae. Am J Trop Med Hyg, 32, 767-71. doi:10.4269/ajtmh.1983.32.767

    Methods are described for the cryopreservation of third-stage larvae of Brugia malayi. Optimum conditions utilized larvae free from the mosquito host frozen at the rate of -1 degree or -0.8 degrees C per min in medium containing 9% dimethyl sulfoxide and 0.004 M polyvinylpyrrolidone. Nonfrozen or thawed larvae were inoculated intraperitoneally into jirds (Meriones unguiculatus), the thawed larvae after cryogenic storage for 5-378 days. In general, the percentage of adult worms recovered at necropsy was comparable between the two groups and ranged from a mean of 6-9% of the larval inoculum. In addition, three of four patas monkeys (Erythrocebus patas) inoculated with thawed B. malayi larvae developed patent infections. The cryopreservation of third-stage larvae of Dipetalonema viteae also is discussed.


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