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Resources » Paper

Spinner WG et al. (2012) Parasitology "Characterization of genes with a putative key role in the parasitic lifestyle of the nematode Strongyloides ratti."

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  • Comments on Spinner WG et al. (2012) Parasitology "Characterization of genes with a putative key role in the parasitic lifestyle of the nematode Strongyloides ratti." (0)

  • Overview

    PMID:
    22717055
    DOI:
    10.1017/S0031182012000637
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00053942

    Spinner WG, Thompson FJ, Emery DC, & Viney ME (2012). Characterization of genes with a putative key role in the parasitic lifestyle of the nematode Strongyloides ratti. Parasitology, 139, 1317-28. doi:10.1017/S0031182012000637

    Parasitic nematodes are significant pathogens of humans and other animals. The molecular and genetic basis of animal parasitism is not yet fully understood. Strongyloides spp. are a genus of gastrointestinal nematodes of which species infect approximately 100200 million people worldwide. S. ratti is a natural parasite of the rat, and a useful and amenable laboratory model. Previous EST and microarray analyses of the S. ratti life cycle have identified genes whose expression was specific, or biased, to the parasitic adult stage, suggesting that they may play a key role in parasitism in this species. Here we have further investigated the expression of these genes (by RT-PCR) throughout the S. ratti life-cycle. We produced recombinant proteins in vitro for a subset of these genes, which were used in Western blot analyses to investigate the distribution of the gene products among different stages of the S. ratti life cycle. We tested the efficacy of these recombinant proteins as anti-S. ratti vaccines. One of the proteins was detected in the excretory/secretory products of the parasitic stages.


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