- page settings
- showhide sidebar
- showhide empty fields
- layout
- (too narrow)
- open all
- close all
- Page Content
- Overview
- External Links
- History
- Referenced
- Tools
- Tree Display
- My WormBase
- My Favorites
- My Library
- Recent Activity
- Comments (0)
history logging is off
Tree Display
My Favorites
My Library
Comments on Morrison, Logan et al. (2017) International Worm Meeting "Sentryn and SAD Kinase link Dense Core Vesicle axonal transport and synaptic capture." (0)
Overview
Morrison, Logan, Edwards, Stacey, Manning, Laura, Stec, Natalia, Richmond, Janet, & Miller, Kenneth (2017). Sentryn and SAD Kinase link Dense Core Vesicle axonal transport and synaptic capture presented in International Worm Meeting. Unpublished information; cite only with author permission.
Dense core vesicles (DCVs) release neuropeptides from the synaptic regions of axons to modulate animal behaviors. Release is preceded by axonal transport of DCVs, followed by their capture in the synaptic region. During transport, motors move DCVs along microtubules bi-directionally, with a bias toward forward transport. Upon capture, DCVs must be protected from the actions of both the forward and reverse motors, known as KIF1A and dynein. The mechanisms that ensure biased forward transport and that protect DCVs from motors after capture are unknown. Here, we use a forward genetic screen to discover two new components of the DCV transport and capture mechanism: SAD Kinase (SAD-1) and a novel protein we named Sentryn (STRN-1). Sentryn is highly expressed in neurons and is conserved in all animals. The name, based on "sentry", reflects its role in standing guard over captured vesicles and protecting them from counter-productive motor activity during transport. A human Sentryn cDNA rescues the worm DCV phenotypes. Our genetic studies show that Sentryn acts cell autonomously with SAD Kinase to optimize the forward transport and synaptic capture of DCVs. Time lapse and high resolution quantitative imaging of single neuron subregions show that Sentryn and SAD Kinase do this by directly or indirectly regulating the interactions of both the KIF1A and Dynein motors with DCVs. These results provide the first evidence for a mechanistic link between DCV transport and synaptic capture by identifying two conserved proteins that function in both processes.
Affiliations:
- University of Illinois at Chicago, Chicago, IL
- Oklahoma Medical Research Foundation, Oklahoma City, OK