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Resources » Paper

Miao, R. et al. (2017) International Worm Meeting "Characterization of Lysosomal Dynamics and Function during C. elegans Larval Development."

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00052071

    Miao, R., Li, Xin, & Wang, Xiaochen (2017). Characterization of Lysosomal Dynamics and Function during C. elegans Larval Development presented in International Worm Meeting. Unpublished information; cite only with author permission.

    C. elegans develops through four consecutive larval stages (L1-L4) separated by molts. During each molt, worms synthesize and secrete a new exoskeleton called cuticle underneath the existing one, followed by their separation (apolysis) and the shed of the old exoskeleton (ecdysis). Lethargus, an inactive sleep-like state characterized by a gradual decrease in general activity and feeding, occurs at the end of each larval stage coinciding with separation of the old exoskeleton from the hypodermis.1, 2 We found that lysosomes, which are labeled by either the lysosomal enzyme NUC-1 or the lysosomal membrane protein LAAT-1, appear as small puncta or thin tubules during larval development. Interestingly, worms at the lethargus stage contain extensive tubular lysosomes in the hypodermis, whereas globular lysosomes are predominant when animals exit lethargus to enter the ecdysis stage. Moreover, EGF overexpression, which induces behavioral quiescence, leads to abundant tubular lysosome formation, whereas physical disturbance of the locomotive quiescence partially disrupts tubular lysosomes formed during lethargus. This indicates a close correlation between tubular lysosome formation and lethargus quiescence. We found that morphology of other intracellular organelles including ER, Golgi, mitochondria and endosomes remain unaltered during lethargus, suggesting that lysosomes are specifically altered at this stage. In addition to morphological changes, we found that lysosome biogenesis and dynamics appear to be up-regulated during lethargus. We are currently investigating how lysosome dynamics and functions are regulated at this specific developmental stage and how they may contribute to the molting process. 1. The cuticle. Antony P. Page and Iain L. Johnstone, 2007, Wormbook. 2. Lethargus is a Caenorhabditis elegans sleep-like state. David M. Raizen et al, 2008, Nature.

    Affiliations:
    - National Institute of Biological Sciences, Beijing, CN
    - Institute of Biophysics, Chinese Academy of Sciences, Beijing, CN


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