Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Keil W et al. (2016) Dev Cell "Long-Term High-Resolution Imaging of Developing C.elegans Larvae with Microfluidics."

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Keil W et al. (2016) Dev Cell "Long-Term High-Resolution Imaging of Developing C.elegans Larvae with Microfluidics." (0)

  • Overview

    PMID:
    28041904
    DOI:
    10.1016/j.devcel.2016.11.022
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00050572

    Keil W, Kutscher LM, Shaham S, & Siggia ED (2016). Long-Term High-Resolution Imaging of Developing C.elegans Larvae with Microfluidics. Dev Cell. doi:10.1016/j.devcel.2016.11.022

    Long-term studies of Caenorhabditis elegans larval development traditionally require tedious manual observations because larvae must move to develop,and existing immobilization techniques either perturb development or are unsuited for young larvae. Here, we present a simple microfluidic deviceto simultaneously follow development of ten C.elegans larvae at high spatiotemporal resolution from hatching to adulthood (3days). Animals grownin microchambers are periodically immobilized bycompression to allow high-quality imaging of even weak fluorescence signals. Using the device,we obtain cell-cycle statistics for C.elegans vulval development, a paradigm for organogenesis. Wecombine Nomarski and multichannel fluorescence microscopy to study processes such as cell-fate specification, cell death, and transdifferentiation throughout post-embryonic development. Finally, we generate time-lapse movies of complex neural arborization through automated image registration. Our technique opens the door to quantitativeanalysis of time-dependent phenomena governing cellular behavior during C.elegans larval development.


    Tip: Seeing your name marked red? Please help us identify you.