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Resources » Paper

Ma H et al. (2016) Neurochem Res "Effects of a Standardized Phenolic-Enriched Maple Syrup Extract on -Amyloid Aggregation, Neuroinflammation in Microglial and Neuronal Cells, and -Amyloid Induced Neurotoxicity in Caenorhabditis elegans."

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  • Comments on Ma H et al. (2016) Neurochem Res "Effects of a Standardized Phenolic-Enriched Maple Syrup Extract on -Amyloid Aggregation, Neuroinflammation in Microglial and Neuronal Cells, and -Amyloid Induced Neurotoxicity in Caenorhabditis elegans." (0)

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    PMID:
    27418278
    DOI:
    10.1007/s11064-016-1998-6
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00049832

    Ma H, DaSilva NA, Liu W, Nahar PP, Wei Z, Liu Y, Pham PT, Crews R, Vattem DA, Slitt AL, Shaikh ZA, & Seeram NP (2016). Effects of a Standardized Phenolic-Enriched Maple Syrup Extract on -Amyloid Aggregation, Neuroinflammation in Microglial and Neuronal Cells, and -Amyloid Induced Neurotoxicity in Caenorhabditis elegans. Neurochem Res. doi:10.1007/s11064-016-1998-6

    Published data supports the neuroprotective effects of several phenolic-containing natural products, including certain fruit, berries, spices, nuts, green tea, and olive oil. However, limited data are available for phenolic-containing plant-derived natural sweeteners including maple syrup. Herein, we investigated the neuroprotective effects of a chemically standardized phenolic-enriched maple syrup extract (MSX) using a combination of biophysical, in vitro, and in vivo studies. Based on biophysical data (Thioflavin T assay, transmission electron microscopy, circular dichroism, dynamic light scattering, and zeta potential), MSX reduced amyloid 1-42 peptide (A1-42) fibrillation in a concentration-dependent manner (50-500g/mL) with similar effects as the neuroprotective polyphenol, resveratrol, at its highest test concentration (63.5% at 500g/mL vs. 77.3% at 50g/mL, respectively). MSX (100g/mL) decreased H2O2-induced oxidative stress (16.1% decrease in ROS levels compared to control), and down-regulated the production of lipopolysaccharide (LPS)-stimulated inflammatory markers (22.1, 19.9, 74.8, and 87.6% decrease in NOS, IL-6, PGE2, and TNF levels, respectively, compared to control) in murine BV-2 microglial cells. Moreover, in a non-contact co-culture cell model, differentiated human SH-SY5Y neuronal cells were exposed to conditioned media from BV-2 cells treated with MSX (100g/mL) and LPS or LPS alone. MSX-BV-2 media increased SH-SY5Y cell viability by 13.8% compared to media collected from LPS-BV-2 treated cells. Also, MSX (10g/mL) showed protective effects against A1-42 induced neurotoxicity and paralysis in Caenorhabditis elegans in vivo. These data support the potential neuroprotective effects of MSX warranting further studies on this natural product.

    Authors: Ma H, DaSilva NA, Liu W, Nahar PP, Wei Z, Liu Y, Pham PT, Crews R, Vattem DA, Slitt AL, Shaikh ZA, Seeram NP


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