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Comments on Hall DH et al. (2015) Methods Mol Biol "Electron Tomography Methods for C. elegans." (0)
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PMID:
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Publication type:
Journal_article
WormBase ID:
WBPaper00048873
Hall DH, & Rice WJ (2015). Electron Tomography Methods for C. elegans. Methods Mol Biol, 1327, 141-58. doi:10.1007/978-1-4939-2842-2_11
Methods for electron tomography of the nematode C. elegans are explained in detail, including a brief introduction to specimen preparation, methods for image collection, and a comparison of several general methods for producing dual-axis tomograms, with or without external fiducial reference objects. New electron tomograms highlight features in software for data display, annotation, and analysis. This chapter discusses the ultrastructural analysis of cells and tissues, rather than molecular studies.
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