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Comments on Head, Brian et al. (2015) International Worm Meeting "The role of GATA and other cell-autonomous transcription factors in mediating C. elegans recovery from acute pathogenic infection." (0)
Overview
Head, Brian, & Aballay, Alejandro (2015). The role of GATA and other cell-autonomous transcription factors in mediating C. elegans recovery from acute pathogenic infection presented in International Worm Meeting. Unpublished information; cite only with author permission.
The host response to acute microbial infection is complex but, at a broad level, involves sensing the pathogen, tolerating pathogen- and host-induced damage, reducing pathogen burden, and, finally, restoring homeostasis. This final phase is critical because if the host cannot restore physiological homeostasis, long-term problems can arise. In humans, these problems manifest in a multitude of disorders including recurrent pathogenic infection, inflammatory bowel diseases (IBDs), and gastritis/duodenitis.We are utilizing a C. elegans acute infection model to study recovery - the final restorative phase in the host response to acute microbial challenge. Our acute infection protocol consists of a short exposure to an intestinal pathogen - either Salmonella enterica or Pseudomonas aeruginosa - followed by antibiotic treatment and a change in food source. In previously published work, we demonstrated that "recovered" animals have a reduced bacterial burden and enhanced survival (similar to unchallenged animals). We also determined that the conserved GATA transcription factor ELT-2 acts in a cell-autonomous manner in the intestine to control the expression of genes that are important for recovery. This transcriptional output consists of genes involved in xenobiotic detoxification, redox regulation, and cellular homeostasis. We are continuining to define the network of cell-autonomous transcription factors (DAF-16, SKN-1) and non-cell autonomous signaling pathways (neuronal circuits) that shape gene expression patterns during recovery. Our recent results will be presented.
Affiliation:
- Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC