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Resources » Paper

Neve, Isaiah et al. (2015) International Worm Meeting "Modified OP50 E. coli expressing dsRNA elicite a robust RNA interference response in C. elegans."

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  • Comments on Neve, Isaiah et al. (2015) International Worm Meeting "Modified OP50 E. coli expressing dsRNA elicite a robust RNA interference response in C. elegans." (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00047249

    Neve, Isaiah, Sowa, Jessica, & Wang, Meng (2015). Modified OP50 E. coli expressing dsRNA elicite a robust RNA interference response in C. elegans presented in International Worm Meeting. Unpublished information; cite only with author permission.

    In the majority of research laboratories using C. elegans as a model, they sustain their animals on the B strain E. coli OP50. This strain is easy to manipulate in the laboratory, and has provided us with a key platform for defining many genetic pathways. Simultaneously, the series of revolutionary realizations which culminated in the 2006 Nobel Prize, quickly allowed the technique of dsRNA feeding to incite RNA interference (RNAi) to become a key technique in many laboratories. Two major resources are available to the RNAi wielding researcher, the Ahringer and Vidal libraries respectively. These libraries house thousands of E. coli clones with unique plasmids expressing dsRNA corresponding to individual C. elegans genes. Countless experimental insights have been facilitated using these resources. However, both libraries are housed in the HT115 E. coli K12 strain background. Several recent studies (Brooks et al., 2009; Soukas et al., 2009; Pang & Curran 2014) have revealed the importance of bacterial environment in eliciting certain genetically induced phenotypes. With the realization that gene-environment interactions may play a key role in the elucidation of genetic pathways, we have generated an OP50 strain capable of dependably housing and robustly expressing dsRNA plasmid vectors. Furthermore, when C. elegans are fed this OP50 strain, they activate a substantial RNAi response against the target gene of interest. We anticipate that this resource will allow the further characterization of gene-environment specific relationships. .

    Affiliation:
    - Molecular and Human Genetics, Baylor College of Medicine, Houston, TX


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