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Resources » Paper

Yan Y et al. (2014) Microsc Microanal "A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens."

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  • Overview

    PMID:
    24444078
    DOI:
    10.1017/S1431927613014037
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00045784

    Yan Y, Jiang L, Aufderheide KJ, Wright GA, Terekhov A, Costa L, Qin K, McCleery WT, Fellenstein JJ, Ustione A, Robertson JB, Johnson CH, Piston DW, Hutson MS, Wikswo JP, Hofmeister W, & Janetopoulos C (2014). A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens. Microsc Microanal, 20, 141-51. doi:10.1017/S1431927613014037

    A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations. The compressor mount is removable and allows easy specimen addition and recovery for later observation. Several customized specimen beds can be incorporated into the base. To demonstrate the capabilities of the device, we have imaged numerous cellular events in several protozoan species, in yeast cells, and in Drosophila melanogaster embryos. We have been able to document previously unreported events, and also perform photobleaching experiments, in conjugating Tetrahymena thermophila.

    Authors: Yan Y, Jiang L, Aufderheide KJ, Wright GA, Terekhov A, Costa L, Qin K, McCleery WT, Fellenstein JJ, Ustione A, Robertson JB, Johnson CH, Piston DW, Hutson MS, Wikswo JP, Hofmeister W, Janetopoulos C


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