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Resources » Paper

Frokjaer-Jensen C et al. (2014) Nat Methods "Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon."

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  • Comments on Frokjaer-Jensen C et al. (2014) Nat Methods "Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon." (0)

  • Overview

    PMID:
    24820376
    DOI:
    10.1038/nmeth.2889
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00045258

    Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, & Jorgensen EM (2014). Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Nat Methods, 11, 529-34. doi:10.1038/nmeth.2889

    We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selection, and the transposon is active in C. elegans isolates and Caenorhabditis briggsae. We used the miniMos transposon to generate six universal Mos1-mediated single-copy insertion (mosSCI) landing sites that allow targeted transgene insertion with a single targeting vector into permissive expression sites on all autosomes. We also generated two collections of strains: a set of bright fluorescent insertions that are useful as dominant, genetic balancers and a set of lacO insertions to track genome position.

    Authors: Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovsky A, Moerman DG, Jorgensen EM


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