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Resources » Paper

Fernandez-Cardenas, Laura P. et al. (2013) International Worm Meeting "The C. elegans ATPase inhibitors MAI-1 and MAI-2 are localized in different cellular compartments."

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  • Comments on Fernandez-Cardenas, Laura P. et al. (2013) International Worm Meeting "The C. elegans ATPase inhibitors MAI-1 and MAI-2 are localized in different cellular compartments." (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00043855

    Fernandez-Cardenas, Laura P., Salinas-Velazquez, L.S., Agredano-Moreno, L.T., Jimenez-Garcia, L.F., Tuena de Gomez Puyou, M., & Navarro, R.E. (2013). The C. elegans ATPase inhibitors MAI-1 and MAI-2 are localized in different cellular compartments presented in International Worm Meeting. Unpublished information; cite only with author permission.

    In mammals, the ATPase inhibitor protein (IF1) regulates the hydrolysis activity of mitochondrial FoF1 ATPase to avoid ATP depletion. Besides mitochondria, this protein can also be found in endothelial and hepatic cell surface where its function is not well understood. In C. elegans there are two homologs for the mitochondrial IF1, MAI-1 and MAI-2. These have been studied in yeast where they both inhibit the FoF1-ATPase. MAI-1 is localized in cytoplasm while MAI-2 in mitochondria. We are studying mai-1 and mai-2 expression and function in C. elegans. To study their expression, we generated transgenic animals, that carry the constructs mai-2::gfp or mCherry::mai-1, by bombardment. We found that mai-2::gfp is expressed in all tissues during all embryonic and larval stages; where it appears to be associated to the mitochondrial network. This expression pattern correlates with its IF1 identity. Unexpectedly, mCherry::mai-1 expression is not associated to the mitochondrial network and instead appears to be diffused in the cytoplasm. While mai-2::gfp is expressed ubiquiously, mCherry::mai-1 was only observed in some cells of the nervous system, hypodermis and intestine during late embryonic, all larval stages and adult animals. mai-1(RNAi) and mai-2(RNAi) animals had normal growth, development and fertility, however have increased physiological apoptosis.

    Affiliations:
    - Biochemistry and Structural Biology, IFC, UNAM, Mexico,D.F., Mexico
    - Cell and Developmental Biology, IFC, UNAM, Mexico,D.F., Mexico
    - Science School, UNAM, MexicoD.F., Mexico


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