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Resources » Paper

Barkoulas, Michalis et al. (2013) International Worm Meeting "Robustness of the vulval cell fate pattern to pathway dosage modulation and cryptic evolution of lin-3 regulatory sequences."

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  • Comments on Barkoulas, Michalis et al. (2013) International Worm Meeting "Robustness of the vulval cell fate pattern to pathway dosage modulation and cryptic evolution of lin-3 regulatory sequences." (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00043579

    Barkoulas, Michalis, Peluffo, Alexandre, & Felix, Marie-Anne (2013). Robustness of the vulval cell fate pattern to pathway dosage modulation and cryptic evolution of lin-3 regulatory sequences presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Biological systems can perform reproducibly to generate invariant outcomes, despite external or internal noise. Over the last few years, the C. elegans vulva has become a model system to explore phenotypic robustness, as the cell fate pattern is highly invariable and the underlying developmental mechanisms well described. Although most of the key molecular factors underpinning vulval formation have now been identified, still very little is understood in quantitative terms. Through experimental perturbations of the two main pathways (EGF and Notch) involved in C. elegans vulval patterning we investigated some quantitative aspects of developmental robustness and system behaviour. We established a dose-response curve for the EGF pathway in the vulva and show that the system can tolerate a four-fold variation in genetic dose of the upstream signalling molecule, lin-3/egf, without phenotypic change in cell fate pattern. Using comparative single molecule FISH hybridisation, we show that levels of lin-3 expression are conserved within C. elegans isolates and C. briggsae, but differ in more distant nematodes, such as C. angaria. We demonstrate that LIN-3 plays a key role in vulval induction in other Caenorhabditis species despite substantial evolution of some cis-regulatory elements required for correct spatiotemporal expression in the C. elegans context.

    Affiliation:
    - IBENS, Paris, France


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