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Comments on Brueggemann, Chantal et al. (2013) International Worm Meeting "Acute odor recognition in AWC neuron of C. elegans after adaptation." (0)
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Brueggemann, Chantal, O'Halloran, Damien, & L'Etoile, Noelle (2013). Acute odor recognition in AWC neuron of C. elegans after adaptation presented in International Worm Meeting. Unpublished information; cite only with author permission.
Odor sensation and chemotaxis are essential responses that allow an organism to locate and move towards food. The paired AWCs neurons sense innately attractive odors such as benzaldehyde, butanone or isoamyl alcohol. However, the organism must also be able to ignore profitless odors. Thus, prolonged exposure of the AWC neurons to benzaldehyde (BA) in the absence of food leads to adaptation of the odor-seeking response. This allows the worm to ignore odors that are not associated with nutrition. Continuous exposure to BA in the absence of food, leads to a relocalization of the cGMP-dependent protein kinase EGL-4 from the cytoplasm into the nucleus. Nuclear EGL-4 is necessary and sufficient to adapt the AWC-mediated response to the odor. We examine the effects of both short- and long-term odor exposure on the acute odor response in AWC and its downstream interneurons by using the GFP-based calcium reporter GCaMP3. We hope to correlate the changes in intercellular calcium flux in response to odor with the subcellular localization of EGL-4 and in parallel with the behavior of the worms.
Affiliations:
- Cell and Tissue Biology, University of California, San Francisco, CA.
- Department of Biological Sciences, George Washington University, Washington, DC.