Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Stevens J et al. (2013) PLoS One "N-glycosylation is required for secretion and mitosis in C. elegans."

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Stevens J et al. (2013) PLoS One "N-glycosylation is required for secretion and mitosis in C. elegans." (0)

  • Overview

    PMID:
    23691084
    DOI:
    10.1371/journal.pone.0063687
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00042421

    Stevens J, & Spang A (2013). N-glycosylation is required for secretion and mitosis in C. elegans. PLoS One, 8, e63687. doi:10.1371/journal.pone.0063687

    N-glycosylation of proteins is an essential process, and N-glucans serve as important beacons in protein folding and ER associated degradation. More importantly, N-glycosylation increases the structural repertoire of proteins because the addition of the N-glucan on proteins will serve as a base for further sugar additions in the Golgi apparatus, and hence complex three-dimensional structures can be build. N-glycosylation is mediated by the ER-resident OST complex, which is essential throughout eukaryotes. Partial knockdown of conserved OST complex members, such as C. elegans RIBO-1, led to an embryonic lethal phenotype. Although the ER morphology was not grossly altered in ribo-1(RNAi) oocytes and embryos, secretion of yolk and of the yolk receptor RME-2 was perturbed in those worms. Perhaps as a consequence of reduced arrival of N-glycosylated proteins at the plasma membrane, cytokinesis occurred less efficiently leading to multinuclear cells. Unexpectedly, we detected a chromosome segregation defect in ribo-1(RNAi) embryos suggesting an essential role of at least one N-glycosylated protein in metaphase-anaphase transition.


    Tip: Seeing your name marked red? Please help us identify you.