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Resources » Paper

Dbrowska-Mas E et al. (2012) Org Biomol Chem "Tyrosine nitration affects thymidylate synthase properties."

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  • Overview

    PMID:
    22072032
    DOI:
    10.1039/c1ob06360j
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00040400

    Dbrowska-Mas E, Fraczyk T, Ruman T, Radziszewska K, Wilk P, Ciesla J, Zielinski Z, Jurkiewicz A, Golos B, Winska P, Walajtys-Rode E, Les A, Niziol J, Jarmula A, Stefanowicz P, Szewczuk Z, & Rode W (2012). Tyrosine nitration affects thymidylate synthase properties. Org Biomol Chem, 10, 323-31. doi:10.1039/c1ob06360j

    Highly purified preparations of thymidylate synthase, isolated from calf thymus, and L1210 parental and FdUrd-resistant cells, were found to be nitrated, as indicated by a specific reaction with anti-nitro-tyrosine antibodies, suggesting this modification to appear endogenously in normal and tumor tissues. Each human, mouse and Ceanorhabditis elegans recombinant TS preparation, incubated in vitro in the presence of NaHCO(3), NaNO(2) and H(2)O(2) at pH 7.5, underwent tyrosine nitration, leading to a V(max)(app) 2-fold lower following nitration of 1 (with human or C. elegans TS) or 2 (with mouse TS) tyrosine residues per monomer. Enzyme interactions with dUMP, meTHF or 5-fluoro-dUMP were not distinctly influenced. Nitration under the same conditions of model tripeptides of a general formula H(2)N-Gly-X-Gly-COOH (X = Phe, Tyr, Trp, Lys, Arg, His, Ser, Thr, Cys, Gly), monitored by NMR spectroscopy, showed formation of nitro-species only for H-Gly-Tyr-Gly-OH and H-Gly-Phe-Gly-OH peptides, the chemical shifts for nitrated H-Gly-Tyr-Gly-OH peptide being in a very good agreement with the strongest peak found in (15)N-(1)H HMBC spectrum of nitrated protein. MS analysis of nitrated human and C. elegans proteins revealed several thymidylate synthase-derived peptides containing nitro-tyrosine (at positions 33, 65, 135, 213, 230, 258 and 301 in the human enzyme) and oxidized cysteine (human protein Cys(210), with catalytically critical Cys(195) remaining apparently unmodified) residues.

    Authors: Dbrowska-Mas E, Fraczyk T, Ruman T, Radziszewska K, Wilk P, Ciesla J, Zielinski Z, Jurkiewicz A, Golos B, Winska P, Walajtys-Rode E, Les A, Niziol J, Jarmula A, Stefanowicz P, Szewczuk Z, Rode W


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