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Resources » Paper

Awani, Awani et al. (2011) International Worm Meeting "Genome wide RNAi screen to study uv1 cell necrosis."

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00039489

    Awani, Awani, Crook, Matt, & Hanna-Rose, Wendy (2011). Genome wide RNAi screen to study uv1 cell necrosis presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Necrosis is more than a phenomenon of uncontrolled cell death. There is increasing evidence to suggest that necrosis is genetically programmed. We are exploiting a new model to investigate necrotic cell death. In worms that lack pnc-1 nicotinamidase, the four uv1 cells die by necrosis (1). We want to investigate why uv1 cells die in pnc-1 mutants and to identify genes that promote or inhibit uv1 cell necrosis. In pnc-1 mutant animals with a uv1 cell specific GFP transgene, we can screen for rescue of uv1 cell necrosis by screening for animals that retain the uv1 GFP signal. We aim to screen using the genome-wide RNAi library and expect to gain insight into how and why uv1 cells die in pnc-1 mutants. We are using a split GFP system to engineer animals with uv1 cell specific GFP expression for this screen (2), and we will discuss progress in engineering the strain and preparing for the screen. Reference: 1. EGF signaling overcomes a uterine cell death associated with temporal mis-coordination of organogenesis within the C. elegans egg-laying apparatus. Li Huang , Hanna-Rose Wendy 2. Antiparallel Leucine Zipper-Directed Protein Reassembly: Application to the Green Fluorescent Protein. Indraneel Ghosh , Andrew D. Hamilton , Lynne Regan.

    Affiliation:
    - Pennsylvania State University, state college, PA.


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