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Resources » Paper

Edgley, Mark et al. (2011) International Worm Meeting "Towards a mutation in every gene: A report from the front lines."

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  • Comments on Edgley, Mark et al. (2011) International Worm Meeting "Towards a mutation in every gene: A report from the front lines." (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00039330

    Edgley, Mark, Barstead, Robert, Mitani, Shohei, & Moerman, Don (2011). Towards a mutation in every gene: A report from the front lines presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Currently the combined efforts of the Barstead, Mitani and Moerman labs have identified over 7,000 deletions in 6,013 genes. About 1/4 of these mutants are inviable as homozygotes. Most of the mutations identified to date have been isolated using the PCR deletion detection protocol but we are also using Comparative Genome Hybridization (CGH) and massively parallel short read sequencing technologies to identify alterations. While the facilities are largely request driven, we have made a concerted effort to identify mutations in a number of large critical genes families, including transcription factors (TF's) and kinases. To date we have identified mutations in close to 900 of the 935 TF's and 381 of the 416 annotated kinases. The efforts of our three facilities when combined with efforts from the community and other more recent results from Deep Sequencing bring the total numbers of genes with either a deletion, a stop codon or a splicing defect to 8,000 genes.

    Affiliations:
    - Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    - Life Sciences Centre, University of British Columbia, Vancouver, BC, Canada
    - Deparment of Zoology, University of British Columbia, Vancouver, BC, Canada
    - Tokyo Women's Medical University School of Medicine, Tokyo, Japan


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