Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Kleemann, Gunnar et al. (2011) International Worm Meeting "Delimiting a polymorphic longevity locus on the left arm of Chromosome IV using traditional and high throughput longevity assays."

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Kleemann, Gunnar et al. (2011) International Worm Meeting "Delimiting a polymorphic longevity locus on the left arm of Chromosome IV using traditional and high throughput longevity assays." (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00038961

    Kleemann, Gunnar, Boom, Joshua, Kruglyak, Leonid, & Murphy, Coleen (2011). Delimiting a polymorphic longevity locus on the left arm of Chromosome IV using traditional and high throughput longevity assays presented in International Worm Meeting. Unpublished information; cite only with author permission.

    A large body of work has been generated describing how aging is altered by changes in signaling pathways. Less well understood is how the known pathways differ across genetically polymorphic natural isolates of C. elegans. In order to explore how the longevity pathways have diverged in wild strains, we have assessed longevity across a panel of CB4856 x N2 recombinant inbred advanced intercross lines (RIAILs) (Rockman and Kruglyak 2009). We facilitated our analysis by developing a high-throughput longevity assay pipeline, "Chronos" to count worms and estimate longevity curves. While N2 and CB4856 longevity were similar, a number of the RIAIL life spans differed significantly from either parent suggesting that parental life span has converged, but hidden genetic variation is revealed when co-adapted alleles are separated. From the linkage data generated in the RIAIL based QTL (quantitative trait loci) analysis we identified a polymorphic region on the left arm of Chromosome IV that confers a longevity difference when the region is isolated on an isogenic N2 background. We have reduced the longevity-linked region from the initial 2.5 Mbp identified in the QTL analysis to <200 kb long region using Near Isogeneic Lines (NILs). The minimal region encompasses likely functional changes in only 8 genes. We are testing the candidate genes using both single gene and fosmid rescue. Additionally, In order to gain a deeper insight into the processes that differ between N2 and the short-lived NIL worms we are conducting a detailed phenotypic analysis of the NIL survival curves. To collect the large number of survival curves required to fully characterize the hazard (instantaneous likelihood of death) function shape, peak time and variation we are using an imaging robot in conjunction with the Chronos image analysis pipeline.

    Affiliation:
    - Lewis-Sigler Institute of Integrative Genomics, Princeton University, New Jersey, USA.


    Tip: Seeing your name marked red? Please help us identify you.