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Resources » Paper

Zhu C et al. (2010) Int J Mol Sci "The lin-4 Gene Controls Fat Accumulation and Longevity in Caenorhabditis elegans."

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  • Comments on Zhu C et al. (2010) Int J Mol Sci "The lin-4 Gene Controls Fat Accumulation and Longevity in Caenorhabditis elegans." (0)

  • Overview

    PMID:
    21614175
    DOI:
    10.3390/ijms11124814
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00038459

    Zhu C, Ji CB, Zhang CM, Gao CL, Zhu JG, Qin DN, Kou CZ, Zhu GZ, Shi CM, & Guo XR (2010). The lin-4 Gene Controls Fat Accumulation and Longevity in Caenorhabditis elegans. Int J Mol Sci, 11, 4814-25. doi:10.3390/ijms11124814

    Previous studies have determined that lin-4, which was the first miRNA to be discovered, controls the timing of cell fate determination and life span in Caenorhabditis elegans. However, the mechanism of lin-4 involvement in these processes remains poorly understood. Fat storage is an essential aspect of the life cycle of organisms, and the function of lin-4 in fat accumulation is not clear. In this study, we showed that the fat content is reduced remarkably in C. elegans lin-4 mutants. Quantitative RT-PCR analysis revealed a considerable decrease in the levels of SBP-1 and OGA-1 mRNA in lin-4 mutants. We also showed that lin-4 mutants have a significantly shorter life span than wild-type worms. DCF assay experiments showed that the reactive oxygen species (ROS) levels increased and mitochondrial DNA (mtDNA) copy number decreased in loss-of-function lin-4 mutants. These mutants also showed attenuation of locomotion. Taken together, our findings suggest that lin-4 may play an important role in regulating fat accumulation and locomotion and that lin-4 may control the life span of C. elegans by mediating ROS production.

    Authors: Zhu C, Ji CB, Zhang CM, Gao CL, Zhu JG, Qin DN, Kou CZ, Zhu GZ, Shi CM, Guo XR


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