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Resources » Paper

Rieckher M et al. (2011) PLoS One "Microscopic optical projection tomography in vivo."

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  • Overview

    PMID:
    21559481
    DOI:
    10.1371/journal.pone.0018963
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00038414

    Rieckher M, Birk UJ, Meyer H, Ripoll J, & Tavernarakis N (2011). Microscopic optical projection tomography in vivo. PLoS One, 6, e18963. doi:10.1371/journal.pone.0018963

    We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms.


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