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Resources » Paper

Pacquelet, Anne et al. (2010) C. elegans: Development and Gene Expression, EMBL, Heidelberg, Germany "The RNA-binding protein LARP-1 promotes oogenesis in a fem-3 dependent manner"

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  • Comments on Pacquelet, Anne et al. (2010) C. elegans: Development and Gene Expression, EMBL, Heidelberg, Germany "The RNA-binding protein LARP-1 promotes oogenesis in a fem-3 dependent manner" (0)

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00036942

    Pacquelet, Anne, Zanin, Esther, & Gotta, Monica (2010). The RNA-binding protein LARP-1 promotes oogenesis in a fem-3 dependent manner presented in C. elegans: Development and Gene Expression, EMBL, Heidelberg, Germany. Unpublished information; cite only with author permission.

    In C. elegans hermaphrodites, a complex regulatory network regulates the switch from sperm to oocyte production. A key player in this regulation is CBC FEM-1 , an ubiquitin ligase formed by the cullin CUL-2, the substrate specific adaptor FEM-1 and the cofactors FEM-2 and FEM-3. CBC FEM-1 allows the degradation of TRA-1, a GLI-family transcription factor that promotes oogenesis. We identified the RNA-binding protein LARP-1 (LA-related protein 1) as a new regulator of sex determination. We found that simultaneous depletion of larp-1 and nos-3 results in germline masculinization. This phenotype is accompanied by a strong reduction in TRA-1 levels. Both the masculinization phenotype and the reduction in TRA-1 levels observed in nos-3;larp-1 mutants require fem-3 activity, suggesting that nos-3 and larp-1 regulate the sperm/oocyte switch by inhibiting fem-3 . Previous data indicated that NOS-3 represses fem-3 translation. Interestingly, we found that fem-3 mRNA levels were reduced in l arp-1 mutants but not in nos-3 mutants, showing that LARP-1 and NOS-3 regulate fem-3 by distinct mechanisms. We are currently investigating whether LARP-1 directly regulates fem-3 .


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