Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Ma H et al. (2009) Biomicrofluidics "A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans."

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Ma H et al. (2009) Biomicrofluidics "A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans." (0)

  • Overview

    PMID:
    20216976
    DOI:
    10.1063/1.3274313
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00036051

    Ma H, Jiang L, Shi W, Qin J, & Lin B (2009). A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans. Biomicrofluidics, 3, 44114. doi:10.1063/1.3274313

    The soil dwelling nematode Caenorhabditis elegans (C. elegans) is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin MPP+ enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research.


    Tip: Seeing your name marked red? Please help us identify you.