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Resources » Paper

Marcela Kokes et al. (2008) Development & Evolution Meeting "GLO-3, a Novel Gut Granule Associated Protein, Regulates Gut Granule Formation in C. elegans"

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  • Comments on Marcela Kokes et al. (2008) Development & Evolution Meeting "GLO-3, a Novel Gut Granule Associated Protein, Regulates Gut Granule Formation in C. elegans" (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00034866

    Marcela Kokes, Beverley Rabbitts, Steven Levitte, & Greg Hermann (2008). GLO-3, a Novel Gut Granule Associated Protein, Regulates Gut Granule Formation in C. elegans presented in Development & Evolution Meeting. Unpublished information; cite only with author permission.

    Caenorhabditis elegans intestinal cells are characterized by the presence of gut granules, lysosome-related organelles that contain autofluorescent and birefringent material. Gut granule formation requires the activity of AP-3 subunits, HOPS, and GLO-1/Rab38, genes whose homologues function in trafficking to lysosomes and lysosome-related organelles. To define the molecular and cellular pathways that function in gut granule biogenesis, we have isolated a collection of mutants that lack and/or mislocalize birefringent material into the embryonic intestinal lumen. The glo (gut granule loss) mutants define at least eight different genes. Here we present our phenotypic and molecular analysis of glo-3. We have cloned glo-3 and find that it encodes a novel predicted membrane protein that is associated with gut granules. Interestingly, glo-3(-) alleles fall into three different phenotypic classes based upon the number of birefringent gut granules present within embryonic intestinal cells. "Strong" glo-3(-) alleles completely lack the compartment while "weak" glo-3(-) alleles contain a reduced number of enlarged gut granules. All glo-3(-) alleles contain a reduced number of gut granules at the adult stage. We are using this characteristic of glo-3(-) alleles to identify other pathways that function in gut granule formation.


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