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Resources » Paper

Diana Dalfo et al. (2008) Development & Evolution Meeting "RNAi screening to identify enhancers of the germline tumor phenotype in C. elegans"

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  • Comments on Diana Dalfo et al. (2008) Development & Evolution Meeting "RNAi screening to identify enhancers of the germline tumor phenotype in C. elegans" (0)

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00034837

    Diana Dalfo, & E. Jane Hubbard (2008). RNAi screening to identify enhancers of the germline tumor phenotype in C. elegans presented in Development & Evolution Meeting. Unpublished information; cite only with author permission.

    There is significant interplay between the control of cell fate decisions and cell proliferation during development, yet the mechanisms underlying this interplay are not well understood. We are using the C. elegans germ line to investigate these mechanisms. C. elegans, germline polarity is established when a subset of previously undifferentiated germ cells differentiate and enter meiosis at a distinct developmental time and position. We have isolated mutants that disrupt the pattern of germline development such that the initial meiotic onset is delayed. The ultimate consequence of this delay is a large mass of proliferating germ cells in the proximal germ line of the adult. This phenotype, proximal proliferation (Pro), is observed with certain alleles of glp-1 (Pepper et al., 2003(a,b)). glp-1 is a receptor of the LIN-12/Notch family, and its activity promotes the proliferative or undifferentiated state and/or inhibits differentiation (meiosis) in the germ line (Austin and Kimble, 1987; Yochem and Greenwald, 1989). We are using a glp-1(Pro) allele as a starting point to identify additional factors that affect early germline proliferation and initial meiotic entry. Our previous data indicate that the distal pair of somatic gonadal sheath cells influence germline proliferation and act as an additional, non-DTC-mediated, mechanism to promote robust larval germline proliferation (Killian and Hubbard, 2005). We also observed that ablation of these cells enhances the glp-1(Pro) phenotype (Killian and Hubbard, 2005). Therefore, to identify the sheath-autonomous proliferation-promoting activity, we are performing candidate and genome-wide RNAi screens for enhancers of the Pro phenotype.


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