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Resources » Paper

Farooqui, Sarfarazhussain et al. (2009) International Worm Meeting "The ROCK homolog LET-502 is temporally and spatially regulated by LIN-12 Notch and LIN-1 ETS during vulval morphogenesis."

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  • Comments on Farooqui, Sarfarazhussain et al. (2009) International Worm Meeting "The ROCK homolog LET-502 is temporally and spatially regulated by LIN-12 Notch and LIN-1 ETS during vulval morphogenesis." (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00034326

    Farooqui, Sarfarazhussain, Rimann, Ivo, & Hajnal, Alex (2009). The ROCK homolog LET-502 is temporally and spatially regulated by LIN-12 Notch and LIN-1 ETS during vulval morphogenesis presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Cell fate specification and morphogenesis are two important aspects of vulval development. After the vulval precursor cells (VPCs) have acquired their fates, the VPCs divide thrice and their descendants undergo migration, elongation and fusion at the vulval midline to form seven toroidal rings stacked one over the other in an invariant fashion (Ranjana Sharma-Kishore et. al, 1999). Recently, genetic approaches have revealed that two parallel pathways regulate vulval morphogenesis. The first pathway requires the SMP-1 Semaphorin ligand, the PLX-1 Plexin receptor and the CED-10 Rac small GTPase, while the second pathway utilizes the MIG-2 Rac small GTPase and its activator UNC-73 GEF (Dalpe et al. 2005; Ranjana S Kishore et al. 2002). Here, we show a role for the ROCK homolog LET-502 during vulval morphogenesis. Maternally rescued let-502(ok1283) animals display defects in vulval toroid formation, with evidence of abnormal cell migration and elongation. The expression of the vulval fate markers was not affected in the let-502(ok1283) animals, suggesting that let-502 does not affect VPC fate specification. Genetic epistasis reveals redundancy of LET-502 with both the SMP-1/PLX-1/CED-10 and UNC-73/MIG-2 signaling pathways. During vulval morphogenesis, let-502 is specifically expressed in the secondary vulval cells. Analysis of the let-502 promoter reveals spatial and temporal regulation during morphogenesis by LIN-12 Notch signaling and the transcription factor LIN-1 ETS. Accordingly, lin-12 notch loss-of-function mutants display defects in toroid formation similar to the defects observed in let-502(ok1283) mutants. Hence, Notch signaling may regulate vulval morphogenesis through the activation of let-502 transcription.


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