Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.
  • page settings
  • hide sidebar
  • show empty fields
  • layout
  • (too narrow)
  • open all
  • close all
Resources » Paper

Pym, Edward et al. (2009) International Worm Meeting "An RNAi screen for transcription factors involved in synaptic function."

  • History

  • Referenced

  • Tree Display

  • My Favorites

  • My Library

  • Comments on Pym, Edward et al. (2009) International Worm Meeting "An RNAi screen for transcription factors involved in synaptic function." (0)

  • Overview

    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00034105

    Pym, Edward, Feliu-Mojer, Monica, Vashlishan, Amy, & Kaplan, Joshua (2009). An RNAi screen for transcription factors involved in synaptic function presented in International Worm Meeting. Unpublished information; cite only with author permission.

    The differential regulation of gene expression, by transcription factors, is essential for both the development and function of the nervous system. During the development of the nervous system, transcription factors play many key roles in a diverse range of decisions, from early cell fate to axon pathfinding and synaptic connectivity. Once the nervous system is ''up and running'' transcription factors take on many functional roles, from responses to environmental stresses such as ischemia, to LTP and synaptic homeostasis. It is now emerging that there are growing classes of neurological disorders characterized by aberrant transcriptional control of proteins in the nervous system, e.g. transcription channelopathies (1). To identify transcription factors that have roles in nervous system we plan to use C.elegans to undertake an RNAi screen, of all annotated transcription factors in the genome, for an altered sensitivity to aldicarb, an acetylcholine esterase inhibitor that causes hypercontraction of muscles in wild type animals. This approached has been successfully used before to isolate many new genes that regulate synaptic function both in traditional EMS mutagenesis screens (2) and in RNAi screens (3). The RNAi screen will be done in both F0 and F1 animals using the nre-1,lin15b strain that allows for enhanced RNAi. We have already performed a small pilot screen, that successfully identified two transcription factors previously un-annotated as having an altered aldicarb sensitivity; mua-1 and cnd-1. (1) Waxman (2001) Nat Rev Neurosci, vol. 2 (9) pp. 652-9. (2) Nguyen, M et al. (1995). Genetics, vol. 140 (2) pp. 527-35. (3) Sieburth, D et al. (2005) Nature, vol. 436 (7050) pp. 510-7.


    Tip: Seeing your name marked red? Please help us identify you.