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Resources » Paper

Nomura, Kazuya et al. (2009) International Worm Meeting "Involvement of glycogenes in cell division and morphogenesis: ."

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    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00033574

    Nomura, Kazuya, Nomura, Kazuko H., Murata, Daisuke, Dejima, Katsufumi, Mizuguchi, Souhei, Ando, Keiko-Gengyo, Mitani, Shohei, Kawasaki, Nana, Yamashita, Katsuko, Ideo, Hiroko, Fukushima, Keiko, Hirabayashi, Yoshio, Kanai, Yoshikatsu, Kwon, Yeon-Dae, Narimatsu, Hisashi, Kitagawa, Hiroshi, Hayashi, Yasuhiro, & Ito, Makoto (2009). Involvement of glycogenes in cell division and morphogenesis: presented in International Worm Meeting. Unpublished information; cite only with author permission.

    Glycogenes are genes essential for synthesis, degradation, modification or recognition of glycoconjugates. Among them are genes of glycosyltransferases involved in synthesis of N-glycans and O-glycans, glycoconjugate degradation enzymes, sulfation-related enzymes, sugar transporters, glycolipid synthesizing and depredating enzymes, GPI-anchor related enzymes as well as various lectins recognizing carbohydrates and related structures. In the present study, to understand the roles of carbohydrates in development and morphogenesis of multicellular organisms, we performed systematic knocking out of glycogenes which are orthologues of human glycogenes. By using sophisticated bioinformatics techniques, about 60% of glycogenes in the worm genome are shown to be orthologues of human glycogenes. For instance, we found 145 predicted orthologues of human glycosyltransferase (GT) genes in the worm genome. In addition to various genes involved in glycosphingolipid or GPI anchor synthesis, we found various sulfation related genes and lectin orthologues in the worm genome. We knocked down all of these gene functions by RNAi and/or TMP/UV deletion mutagenesis and found at least 10% of them are essential and over 30% of these genes show various phenotypes when knocked down or knocked out. Larval lethality and ER stress phenotypes were observed in sulfation related gene KO experiments (PAPS synthase pps-1 or PAPS transporter genes) and ER stress phenotypes were observed when hut-1 gene was knocked out. Especially intriguing phenotype was the abnormal cytokinesis in early embryonic division observed in chondroitin related gene KO experiments (chondroitin synthase: sqv-5, chondroitin polymerizing factor: mig-22/pfc-1 or cpg-1 and cpg-2 double KO). Meiotic division abnormal phenotypes were also observed in chondroitin related gene KO experiments, and we also found that various other glycogenes are involved in progression of meiotic cell cycle. These results indicate that genes involved in glycosylation are playing essential roles in embryonic cell division as well as in meiotic cell division.

    Authors: Nomura, Kazuya, Nomura, Kazuko H., Murata, Daisuke, Dejima, Katsufumi, Mizuguchi, Souhei, Ando, Keiko-Gengyo, Mitani, Shohei, Kawasaki, Nana, Yamashita, Katsuko, Ideo, Hiroko, Fukushima, Keiko, Hirabayashi, Yoshio, Kanai, Yoshikatsu, Kwon, Yeon-Dae, Narimatsu, Hisashi, Kitagawa, Hiroshi, Hayashi, Yasuhiro, Ito, Makoto


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