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Resources » Paper

O'Toole E et al. (2009) Methods Mol Biol "Electron tomography of microtubule end-morphologies in C. elegans embryos."

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  • Comments on O'Toole E et al. (2009) Methods Mol Biol "Electron tomography of microtubule end-morphologies in C. elegans embryos." (0)

  • Overview

    PMID:
    19475386
    DOI:
    10.1007/978-1-60327-993-2_8
    Status:
    Publication type:
    Journal_article
    WormBase ID:
    WBPaper00033404

    O'Toole E, & Muller-Reichert T (2009). Electron tomography of microtubule end-morphologies in C. elegans embryos. Methods Mol Biol, 545, 135-44. doi:10.1007/978-1-60327-993-2_8

    In this chapter we describe the preparation of early mitotic C. elegans embryos for the tomographic reconstruction of end-morphologies of spindle microtubules. Early embryos are prepared by high-pressure freezing and freeze-substitution for thin-layer embedding in Epon/Araldite. We further describe data acquisition, tomographic reconstruction, and 3-D modeling of microtubules in serially sectioned mitotic spindles. The presented techniques are applicable to other model systems.


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