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Resources » Paper

Valerie Robert et al. (2008) European Worm Meeting "Repair of a DNA double strand break triggered by Mos1 excision in the germ line of Caenorhabditis elegans."

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  • Comments on Valerie Robert et al. (2008) European Worm Meeting "Repair of a DNA double strand break triggered by Mos1 excision in the germ line of Caenorhabditis elegans." (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00033364

    Valerie ROBERT, Erik Jorgensen, & Jean-Louis BESSEREAU (2008). Repair of a DNA double strand break triggered by Mos1 excision in the germ line of Caenorhabditis elegans presented in European Worm Meeting. Unpublished information; cite only with author permission.

    Transposable elements (TE) are widely acknowledged as drivers of genome. evolution. To analyze the potential mutagenicity of TE excision, we. investigated the nature of the modifications that the C. elegans genome. undergoes during the repair of a DNA double-strand break (DSB) triggered by. TE mobilization.. Mos1 is a Drosophila transposon which can be experimentally mobilized. in C. elegans. We took advantage of this system to trigger single DSBs at. defined loci and study their repair. We demonstrated that DSB are repaired. by pathways relying on both end-joining and homologous recombination.. Specifically, detailed analysis of germ line repair events showed that most. of the Mos1-induced DSB are repaired by recombination using the homologous. chromosome as a repair template and that these events can be associated. with crossovers. Recombination at homologous ectopic sites was also. observed at a low but significant frequency. In addition, end-joining. events and intrachromosomal recombination were also detected. Together,. these results demonstrate that TE can induce heritable gross chromosomal. rearrangements as well as point mutations and small deletions or insertions. when they transpose in the germ line of wild-type C. elegans.. These data were used to develop MosTIC (which stands for "Mos1 induced. transgene instructed gene conversion"), a technique based on homologous. recombination that enables genomic engineering in C. elegans. This. technique will be presented in details.


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